PolySia: A new bioidentical structural substance  
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     ::  PROJECT      
 
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BIOLOGICAL CHARACTERISATION


Biological Characterisation

 


Scheme of screening system for material testing
Bruns et al. 2007, Stark et al. 2007

 


Bioreactor prototype for tissue engineering

 

Screening with cell lines (static)


1. Viability, Adhesion, Morphology

 

 

2. Proliferation, Differentiation

 

 

Cultivation in a bioreactor (dynamic)

 

 

In vitro screening using primary Neurons & Schwann cells


Sensory Neurons
from
dorsal root ganglia
 
Viability,
Adhesion,
Morphology,
Neurite outgrowth

Neonatal rat Schwann cells on  
PolySia-nanofibers + Poly-l-Lysine coating  

Schwann cell growth influenced by fiber orientation  

Change in growth direction at interrupted fibers  

 

 


 Contact

 


 References

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


 

 

The goal of WP 6 is the biological in vitro characterisation of the modified polySia materials. The parameters which will be evaluated include viability and morphology through the application of the developed screening system using model cell lines (PC 12 and Hep-G2) and Schwann cells. Furthermore, different bioreactor systems for dynamic cultivations will be developed and optimized. The bioreactors will be designed for testing high numbers of different materials under controlled and reproducible conditions. Therefore, the bioreactors will be coupled to a control unit, which will be developed for data acquisition. Sensors for oxygen, pH value, and temperature will be selected from commercially available products, and adapted and integrated into the bioreactor system. The measured values lead to the realization of optimal cultivation conditions.

The biological effects of PolySia and PolySia-based materials on cultures of primary neurons and peripheral glia (Schwann cells) is evaluated in vitro. Evaluated neurons are: sensory dorsal root ganglia neurons from neonatal rats, motoneurons from embryonic rat spinal cord, or mesencephalic neural progenitor cells. Schwann cells are cultured from sciatic nerves of neonatal or adult rats but also from peripheral nerves of human donors. Cultures are analysed with regard to cell adhesion, viability, proliferation rates, as well as cell differentiation (neurite outgrowth) on the different PolySia-based substrata.



   













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