PolySia: Eine neue bioidentische Gerüstsubstanz  
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Projekt-relevante Veröffentlichungen

  1. Bice,I., Celik,H., Wolff,C., Beutel,S., Zahid,M., Hitzmann,B., Rinas,U., Kasper,C., Gerardy-Schahn,R., Scheper,T. (2013). Downstream processing of high chain length polysialic acid using membrane adsorbers and clay minerals for application in tissue engineering. Engineering in Life Sciences 13:2, 140-8.

  2. Chen,R., John,J., Rode,B., Hitzmann,B., Gerardy-Schahn,R., Kasper,C., Scheper,T. (2011). Comparison of polysialic acid production in Escherichia coli K1 during batch cultivation and fed-batch cultivation applying two different control strategies. Journal of biotechnology, 154:4, 222-9.

  3. Assmann,U., Szentivanyi,A., Stark,Y., Scheper,T., Berski,S., Dräger,G., Schuster,R.H. (2010). Journal of Materials Science: Materials in Medicine 21, 2115-2124.

  4. Schmitte,R., Tipold,A., Stein,V.M., Schenk,H., Flieshardt,C., Grothe,C., Haastert,K. (2010). Genetically modified canine Schwann cells - in vitro and in vivo evaluation of their suitability for periheral nerve tissue engineering. J Neurosci Methods, 186, 202-208.

  5. Schulz, E.-C., Dickmanns, A., Urlaub, H., Schmitt, A., Mühlenhoff, M., Stummeyer, K., Schwarzer, D., Gerardy-Schahn, R. and Ficner, R. (2010) Crystal structure of a novel intramolecular chaperone mediating triple ß-helix folding. Nat. Struct. Mol. Biol., 17, 210-5.

  6. Schulz EC, Schwarzer D, Frank M, Stummeyer K, Mühlenhoff M, Dickmanns A, Gerardy-Schahn R*, Ficner R.* (2010). Structural basis for recognition and cleavage of polysialic acid by the bacteriophage K1F tailspike protein endoNF. J. Mol. Biol., 397, 341-351.

  7. Steinhaus,S., Stark,Y., Bruns,S., Haile,Y., Scheper,T., Grothe,C., Behrens,P. (2010) Polysialic acid immobilized on silanized glass surfaces: a test case for its use as a biomaterial for nerve regeneration. J Mater Sci: Mater Med 21, 1371-1378.

  8. Su, Y., Kasper, C., Kirschning, A., Dräger, G., Berski, S. (2010). Synthesis of new polysialic acid derivatives. Macromolecular Bioscience, 10(9),1028-1033.

  9. Schulz, E.-C., Neumann, P., Gerardy-Schahn, R., Sheldrick, G.M., and Ficner, R. (2009) Crystal Structure Analysis of EndosialidaseNF at 0.98 Å Resolution. Acta Crystallogr. D Biol. Crystallogr., 66(Pt 2), 176-180.

  10. Schwarzer,D., Stummeyer,K., Haselhorst,T., Freiberger,F., Rode,B., Grove,M., Scheper,T., von Itzstein,M., Mühlenhoff,M., and Gerardy-Schahn,R. (2009). Proteolytic release of the intramolecular chaperone domain confers processivity to endosialidase F. J. Biol. Chem., 284, 9465-9474.

  11. Galuska,S.P., Geyer,R., Gerardy-Schahn,R., Mühlenhoff,M., and Geyer,H. (2008). Enzyme-dependent variations in the polysialylation of the neural cell adhesion molecule (NCAM) in vivo. J. Biol. Chem., 283, 17-28.

  12. Haastert, K., Schaper-Rinkel, J., Haile, Y., Rode, B., Gerardy-Schahn, R., Scheper, T., Grothe, C. (2008) In vivo evaluation of polysialic acid (polySia) as part of bioengineered peripheral nerve transplants. 327.14/C23. 2008 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2008. Online.

  13. Berski,S., van Bergeijk,J., Schwarzer,D., Stark,Y., Kasper,C., Scheper,T., Grothe,C., Gerardy-Schahn,R., Kirschning,A., and Dräger,G. (2008). Synthesis and Biological Evaluation of a Polysialic Acid-Based Hydrogel as Enzymatically Degradable Scaffold Material for Tissue Engineering. Biomacromolecules, 9, 2353-2359.

  14. Haastert, K., Schaper-Rinkel, J.,Haile, Y., Rode, B., Gerardy-Schahn, R., Scheper T., Grothe, C. (2008) In vivo evaluation of polysialic acid (polysia) as part of bioengineered peripheral nerve transplants. Oral Presenation. Annual Workshop 2008 German Anatomical Society.

  15. Haastert-Talini,K., Schaper-Rinkel,J., Schmitte,R., Rode,B., Mühlenhoff,M., Schwarzer,D., Dräger,G., Su,Y., Scheper,T., Gerardy-Schahn,R., and Grothe,C. (2010). In vivo evaluation of polysialic acid as part of tissue engineered nerve transplants. Tissue Eng. Part A, 16, 3085-3098.

  16. Haile, Y., Berski, S., Dräger, G., Nobre, A., Stummeyer, K., Gerardy-Schahn, R. and Grothe, C. (2008) The effect of modified polysialic acid based hydrogels on the adhesion and viability of primary neurons and glial cells. Biomaterials, 29, 1880-1891.

  17. Haile, Y., Haastert, K., Cesnulevicius, K., Stummeyer, K., Timmer, M., Berski, S., Dräger, G., Gerardy-Schahn, R. and Grothe, C. (2007) Culturing of glial and neuronal cells on polysialic acid. Biomaterials, 28, 1163-1173.

  18. Hartlieb,S., Günzel,A., Gerardy-Schahn,R., Münster-Kühnel,A.K., Kirschning,A., and Dräger,G. (2008). Chemoenzymatic synthesis of CMP-N-acetyl-7-fluoro-7-deoxy-neuraminic acid. Carbohydr. Res., 343, 2075-2082.

  19. Oltmann-Norden,I., Galuska,S.P., Hildebrandt,H., Geyer,R., Gerardy-Schahn,R., Geyer,H., and Mühlenhoff,M. (2008). Impact of the polysialyltransferases ST8SiaII and ST8SiaIV on polysialic acid synthesis during postnatal mouse brain development. J. Biol. Chem., 283, 1463-1471.

  20. Stark,Y., Bruns,S., Stahl,F., Kasper,C., Wesemann,M., Grothe,C., Scheper,T. (2008). A study on polysialic acid as a biomaterial for cell culture applications. Journal of Biomedical Materials Research Part A, 85A(1), 1-13.

  21. Bruns,S., Stark,Y., Wieland,M., Stahl,F., Kasper,C., Scheper,T. (2007). Fast and efficient screening system for new biomaterials in tissue engineering: a model for peripheral nerve regeneration. J Biomed Mater Res A, 81(3), 736-747.

  22. Bruns,S., Stark,Y., Roker,S., Wieland,M., Drager,G., Kirschning,A., Stahl,F., Kasper,C., Scheper,T. (2007). Collagen biomaterial doped with colominic acid for cell culture applications with regard to peripheral nerve repair. J Biotechnol 131(3), 335-345.

  23. Freiberger,F., Claus,H., Günzel,A., Oltmann-Norden,I., Vionnet,J., Mühlenhoff,M., Vogel,U., Vann,W.F., Gerardy-Schahn,R., and Stummeyer,K. (2007). Biochemical characterization of a Neisseria meningitidis polysialyltransferase reveals novel functional motifs in bacterial sialyltransferases. Mol. Microbiol., 65, 1258-1275.

  24. Haselhorst,T., Münster-Kühnel,A.K., Oschlies,M., Tiralongo,J., Gerardy-Schahn,R., and von Itzstein,M. (2007). Direct detection of ligand binding to Sepharose-immobilised protein using saturation transfer double difference (STDD) NMR spectroscopy. Biochem. Biophys. Res. Commun., 359, 866-870.

  25. Leiman,P.G., Battisti,A.J., Bowman,V.D., Stummeyer,K., Mühlenhoff,M., Gerardy-Schahn,R., Scholl,D., and Molineux,I.J. (2007). The structures of bacteriophages K1E and K1-5 explain processive degradation of polysaccharide capsules and evolution of new host specificities. J. Mol. Biol., 371, 836-849.

  26. Schwarzer,D., Stummeyer,K., Gerardy-Schahn,R., and Mühlenhoff,M. (2007). Characterization of a novel intramolecular chaperone domain conserved in endosialidases and other bacteriophage tail spike and fiber proteins. J. Biol. Chem., 282, 2821-2831.

  27. Haselhorst,T., Stummeyer,K., Mühlenhoff,M., Schaper,W., Gerardy-Schahn,R., and von Itzstein,M. (2006). Endosialidase NF appears to bind polySia DP5 in a helical conformation. Chembiochem., 7, 1875-1877.

  28. Stummeyer,K., Schwarzer,D., Claus,H., Vogel,U., Gerardy-Schahn,R., and Mühlenhoff,M. (2006). Evolution of bacteriophages infecting encapsulated bacteria: lessons from Escherichia coli K1-specific phages. Mol. Microbiol., 60, 1123-1135.

  29. Stummeyer,K., Dickmanns,A., Mühlenhoff,M., Gerardy-Schahn,R., and Ficner,R. (2005). Crystal structure of the polysialic acid-degrading endosialidase of bacteriophage K1F. Nat. Struct. Mol. Biol., 12, 90-96.

















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